A retroviral packaging cell line for pseudotype vectors based on glioma-infiltrating progenitor cells.

Gene therapy of malignant glioma with retroviral vectors and tumor- infiltrating progenitor cells

Highly efficient gene transfer into baboon marrow repopulating cells using GALV-pseudotype oncoretroviral vectors produced by human packaging cells.

Vector-containing medium harvested from murine packaging cell lines has been shown to contain factors that can negatively influence the transduction and maintenance of hematopoietic stem cells. Thus, we generated a human packaging cell line with a gibbon ape leukemia virus pseudotype (Phoenix-GALV), and we evaluated vectors produced by Phoenix-GALV for their ability to transduce hematopoietic p...

Ectopic Expression of Embryo/Cancer Sequence A (ECSA) in KYSE-30 Cell Line Using Retroviral System

Background Human preimplantation embryonic cells share many similarities with cancer cells such as ability to self-renew, unlimited proliferation and maintenance of the undifferentiated state. Embryo-cancer sequence A (ECSA), also known as developmental pluripotency associated-2 (DPPA2), is a cancer testis antigen (CTA) with unclear biological function yet. Objective: CTAs are expressed normal...

Gene Therapy for Malignant Gliomas

Retroviruses are enveloped RNA viruses that possess the ability to integrate into the host cell genome by transcribing DNA from their RNA template via viral reverse transcriptase. The transcribed viral DNA is then integrated into the host cell genome nonspecifically. There is relative specificity for tumors because, except for lentiviruses, retroviral DNA preferentially integrates into the geno...

Avian reticuloendotheliosis virus strain A and spleen necrosis virus do not infect human cells.

Spleen necrosis virus (SNV) and Reticuloendotheliosis virus strain A (REV-A) belong to the family of reticuloendotheliosis viruses and are 90% sequence related. SNV-derived retroviral vectors produced by the REV-A-based D17.2G packaging cell line were shown to infect human cells (H.-M. Koo, A. M. C. Brown, Y. Ron, and J. P. Dougherty, J. Virol. 65:4769-4776, 1991), while similar vectors produce...